cells/mL = average count per square × dilution factor × 10⁴. Count cells from hemocytometer readings.
Cell Counts per Square (up to 5)
Assumes each count is for a large 1 mm x 1 mm corner square (volume 0.1 mm³ = 10⁻⁴ mL) of a standard Neubauer-type chamber with a 0.1 mm depth. The dilution factor is the total dilution of the counted sample (for example 2 for a 1:1 mix with trypan blue). For reliable counts count at least about 100 cells in total (counts under 10 per square, or over 100, are unreliable). Research use only.
Related Tools
Most Searched Today
Frequently Asked Questions
No. Hemocytometer Count is free, with no signup required, and nothing you enter leaves your browser.
Hemocytometer Count is built on published formulas and reference ranges from established health sources. We revisit it periodically as guidance evolves; the "Last reviewed" date above shows when that last happened. If something looks wrong, tell us with the feedback button in the bottom-right corner.
No. Hemocytometer Count gives you a general estimate based on the inputs you provide, not a diagnosis. Existing conditions, medications, and individual history all change what a number actually means for you. Treat this as a starting point for a conversation with a doctor or other qualified health professional, not a substitute for one.