C_n = C_0 × (V_transfer/V_total)^n. Calculate concentrations across serial dilution steps.
Transfer volume is the sample moved into each tube; total volume is the final volume of that tube (sample + diluent). Example: 1 mL into 9 mL diluent = transfer 1, total 10 (1:10 per step). Assumes ideal mixing and volumetric accuracy.
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Serial Dilution Calc is built on published formulas and reference ranges from established health sources. We revisit it periodically as guidance evolves; the "Last reviewed" date above shows when that last happened. If something looks wrong, tell us with the feedback button in the bottom-right corner.
No. Serial Dilution Calc gives you a general estimate based on the inputs you provide, not a diagnosis. Existing conditions, medications, and individual history all change what a number actually means for you. Treat this as a starting point for a conversation with a doctor or other qualified health professional, not a substitute for one.